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anti muc2  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology anti muc2
    Anti Muc2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 554 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+muc2/Mucin+2+Antibody/pmc13041762-213-52-55
    Average 96 stars, based on 554 article reviews
    anti muc2 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Immunofluorescence:

    Article Title: Hepatitis E virus replication is maintained in proliferative cells within the intestinal crypt.
    Article Snippet: .. The following antibodies were used for immunofluorescence staining at indicated concentrations: polyclonal anti- ORF2 HEV (1:1000, a gift from R. Ulrich); anti- VP3 HAV (2 μg/ml; Invitrogen, ref. MA17371); anti- CDX2 (1:100; Abcam, ref. AB157524), anti- CHGA (1.75 μg/ ml; Abcam, ref. AB15160); anti- Ki67 (2 μg/ml; Santa Cruz Biotechnology, ref. sc23900); anti- LYZ (1:100; Invitrogen, ref. PA1- 29680); anti- MUC2 (2 μg/ml; Santa Cruz Biotechnology, ref. sc- 515032); anti- VIL (0.625 μg/ml; BD, ref. 10358); anti- OLFM4 (1:100; Cell Signaling Technology, ref. 14369 T); anti- LGR5 (12.1 μg/ml; GeneTex, ref. GTX130204); and Alexa Fluor–conjugated secondary antibodies 488, 594, and 647 (2 μg/ml; Thermo Fisher Scientific). .. Hoechst 33342 (1:1000, Thermo Fisher Scientific) was used for nuclei staining, and Phalloidin- iFluor 647 (0.1×; Abcam, ref. AB176759) was used as a cell junction marker.

    Article Title: Decoding the role of the intestinal epithelium in hepatitis E virus infection using a human organoid prototype of "gut-liver" axis.
    Article Snippet: Ribavirin and Human IFNα were purchased from Sigma. .. The antiHEV ORF2 protein antibody (EMD Millipore), anti-dsRNA antibody (Scicons J2), β-actin antibody (Santa Cruz Biotechnology), anti-EpCAM antibody (Abcam), anti-Villin (Santa Cruz), anti-muc2 (Santa Cruz), anti-CHGA (Bio-Connect BV), anti-rabbit IgG (H + L, Alexa Fluor® 488), anti-mouse IgG (H + L, Alexa Fluor® 594) and DAPI (4, 6-diamidino-2phenylindole; Invitrogen) were used for immunofluorescence staining. ..

    Article Title: Hepatitis E virus replication is maintained in proliferative cells within the intestinal crypt
    Article Snippet: .. The following antibodies were used for immunofluorescence staining at indicated concentrations: polyclonal anti-ORF2 HEV (1:1000, a gift from R. Ulrich); anti-VP3 HAV (2 μg/ml; Invitrogen, ref. MA1-7371); anti-CDX2 (1:100; Abcam, ref. AB157524 ), anti-CHGA (1.75 μg/ml; Abcam, ref. AB15160); anti-Ki67 (2 μg/ml; Santa Cruz Biotechnology, ref. sc23900); anti-LYZ (1:100; Invitrogen, ref. PA1-29680); anti-MUC2 (2 μg/ml; Santa Cruz Biotechnology, ref. sc-515032); anti-VIL (0.625 μg/ml; BD, ref. 10358); anti-OLFM4 (1:100; Cell Signaling Technology, ref. 14369 T); anti-LGR5 (12.1 μg/ml; GeneTex, ref. GTX130204); and Alexa Fluor–conjugated secondary antibodies 488, 594, and 647 (2 μg/ml; Thermo Fisher Scientific). .. Hoechst 33342 (1:1000, Thermo Fisher Scientific) was used for nuclei staining, and Phalloidin-iFluor 647 (0.1×; Abcam, ref. AB176759 ) was used as a cell junction marker.

    Staining:

    Article Title: Hepatitis E virus replication is maintained in proliferative cells within the intestinal crypt.
    Article Snippet: .. The following antibodies were used for immunofluorescence staining at indicated concentrations: polyclonal anti- ORF2 HEV (1:1000, a gift from R. Ulrich); anti- VP3 HAV (2 μg/ml; Invitrogen, ref. MA17371); anti- CDX2 (1:100; Abcam, ref. AB157524), anti- CHGA (1.75 μg/ ml; Abcam, ref. AB15160); anti- Ki67 (2 μg/ml; Santa Cruz Biotechnology, ref. sc23900); anti- LYZ (1:100; Invitrogen, ref. PA1- 29680); anti- MUC2 (2 μg/ml; Santa Cruz Biotechnology, ref. sc- 515032); anti- VIL (0.625 μg/ml; BD, ref. 10358); anti- OLFM4 (1:100; Cell Signaling Technology, ref. 14369 T); anti- LGR5 (12.1 μg/ml; GeneTex, ref. GTX130204); and Alexa Fluor–conjugated secondary antibodies 488, 594, and 647 (2 μg/ml; Thermo Fisher Scientific). .. Hoechst 33342 (1:1000, Thermo Fisher Scientific) was used for nuclei staining, and Phalloidin- iFluor 647 (0.1×; Abcam, ref. AB176759) was used as a cell junction marker.

    Article Title: Decoding the role of the intestinal epithelium in hepatitis E virus infection using a human organoid prototype of "gut-liver" axis.
    Article Snippet: Ribavirin and Human IFNα were purchased from Sigma. .. The antiHEV ORF2 protein antibody (EMD Millipore), anti-dsRNA antibody (Scicons J2), β-actin antibody (Santa Cruz Biotechnology), anti-EpCAM antibody (Abcam), anti-Villin (Santa Cruz), anti-muc2 (Santa Cruz), anti-CHGA (Bio-Connect BV), anti-rabbit IgG (H + L, Alexa Fluor® 488), anti-mouse IgG (H + L, Alexa Fluor® 594) and DAPI (4, 6-diamidino-2phenylindole; Invitrogen) were used for immunofluorescence staining. ..

    Article Title: Hepatitis E virus replication is maintained in proliferative cells within the intestinal crypt
    Article Snippet: .. The following antibodies were used for immunofluorescence staining at indicated concentrations: polyclonal anti-ORF2 HEV (1:1000, a gift from R. Ulrich); anti-VP3 HAV (2 μg/ml; Invitrogen, ref. MA1-7371); anti-CDX2 (1:100; Abcam, ref. AB157524 ), anti-CHGA (1.75 μg/ml; Abcam, ref. AB15160); anti-Ki67 (2 μg/ml; Santa Cruz Biotechnology, ref. sc23900); anti-LYZ (1:100; Invitrogen, ref. PA1-29680); anti-MUC2 (2 μg/ml; Santa Cruz Biotechnology, ref. sc-515032); anti-VIL (0.625 μg/ml; BD, ref. 10358); anti-OLFM4 (1:100; Cell Signaling Technology, ref. 14369 T); anti-LGR5 (12.1 μg/ml; GeneTex, ref. GTX130204); and Alexa Fluor–conjugated secondary antibodies 488, 594, and 647 (2 μg/ml; Thermo Fisher Scientific). .. Hoechst 33342 (1:1000, Thermo Fisher Scientific) was used for nuclei staining, and Phalloidin-iFluor 647 (0.1×; Abcam, ref. AB176759 ) was used as a cell junction marker.

    Incubation:

    Article Title: Advanced human organoid-on-chip with physiological cellular complexity reveals bidirectional secretion patterns
    Article Snippet: .. Then 609 primary antibody solution was added, anti-ZO-1 (1:100; Life Technologies, 61-7300), anti-610 Occludin (1:100; Life Technologies, 33-1500), anti-MUC2 (1:200; Santa Cruz Biotechnology, 611 sc-515032), anti-KI67 (1:200; abcam, ab15580), and incubated for 2 h at room temperature. .. 612 After washing secondary antibody, anti-rabbit-488 (1:500; Invitrogen, A11034) or anti-mouse-613 568 (1:500; Invitrogen, A11031), Phalloidin-643 (1:500; ATTO TEC, AD 643-81) and Hoechst 614 33342 (1:500; Fisher Scientific, H3570) were added and incubated for 1 h at room temperature.

    Article Title: Advanced human organoid-on-chip with physiological cellular complexity reveals bidirectional secretion patterns
    Article Snippet: .. Then primary antibody solution was added, anti-ZO-1 (1:100; Life Technologies, 61–7300), anti-Occludin (1:100; Life Technologies, 33–1500), anti-MUC2 (1:200; Santa Cruz Biotechnology, sc-515032), anti-KI67 (1:200; abcam, ab15580), and incubated for 2 h at room temperature. .. After washing secondary antibody, anti-rabbit-488 (1:500; Invitrogen, A11034) or anti-mouse-568 (1:500; Invitrogen, A11031), Phalloidin-643 (1:500; ATTO TEC, AD 643-81) and Hoechst 33342 (1:500; Fisher Scientific, H3570) were added and incubated for 1 h at room temperature.

    Chromatin Immunoprecipitation:

    Article Title: Substitution of polysorbates by plant-based emulsifiers: impact on vitamin D bioavailability and gut health in mice
    Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Mouse inflamation Panel Detection Antibodies from the kit LEGENDplex Mouse Inflammation Panel (13-plex) with V-botton Plate, biolegend, (Catalog number 740446) Anti-mouse IgG Horseradish Peroxidase linked (CliniScience, 01017-05) 3 nature portfolio | reporting sum m ary April 2023 Goat anti-mouse IgG Fab-HRP (Southern Biotech, Cliniscience 1015-05) anti-MUC2, Santa Cruz Biotechnology, Cat#sc-15334; AB_2146667 anti-VVA, Vector Labs, B-1235-2 Mouse antibodies from the kit Mouse Lipocalin-2/NGAL DuoSet ELISA, Bio-Techne SAS, Catalog #: DY1857 Validation Mouse inflamation Panel Detection Antibodies :Validated by the manufacturer Anti-mouse IgG Horseradish Peroxidase linked : Validated by manufacturer Goat anti-mouse IgG Fab-HRP : Validated by manufacturer anti-MUC2 and anti-VVA validated by manufacturer and in the lab for specific conditions Mouse antibodies from the kit Mouse Lipocalin-2/NGAL DuoSet ELISA, validated by manufacturer Eukaryotic cell lines Policy information about cell lines and Sex and Gender in Research Cell line source(s) Caco2-TC7, HEK-TLR4 and HEK-TLR5 Authentication For Caco2-TC7 Morphology check by microscope, growth curve analysis, control number of passages For HEK-TLR4 and HEK-TLR5 Indirect authentication through the use of selection agents and the creation of a standard range to validate TLR4 or TLR5 expression. ..

    Flow Cytometry:

    Article Title: Substitution of polysorbates by plant-based emulsifiers: impact on vitamin D bioavailability and gut health in mice
    Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Mouse inflamation Panel Detection Antibodies from the kit LEGENDplex Mouse Inflammation Panel (13-plex) with V-botton Plate, biolegend, (Catalog number 740446) Anti-mouse IgG Horseradish Peroxidase linked (CliniScience, 01017-05) 3 nature portfolio | reporting sum m ary April 2023 Goat anti-mouse IgG Fab-HRP (Southern Biotech, Cliniscience 1015-05) anti-MUC2, Santa Cruz Biotechnology, Cat#sc-15334; AB_2146667 anti-VVA, Vector Labs, B-1235-2 Mouse antibodies from the kit Mouse Lipocalin-2/NGAL DuoSet ELISA, Bio-Techne SAS, Catalog #: DY1857 Validation Mouse inflamation Panel Detection Antibodies :Validated by the manufacturer Anti-mouse IgG Horseradish Peroxidase linked : Validated by manufacturer Goat anti-mouse IgG Fab-HRP : Validated by manufacturer anti-MUC2 and anti-VVA validated by manufacturer and in the lab for specific conditions Mouse antibodies from the kit Mouse Lipocalin-2/NGAL DuoSet ELISA, validated by manufacturer Eukaryotic cell lines Policy information about cell lines and Sex and Gender in Research Cell line source(s) Caco2-TC7, HEK-TLR4 and HEK-TLR5 Authentication For Caco2-TC7 Morphology check by microscope, growth curve analysis, control number of passages For HEK-TLR4 and HEK-TLR5 Indirect authentication through the use of selection agents and the creation of a standard range to validate TLR4 or TLR5 expression. ..

    Magnetic Resonance Imaging:

    Article Title: Substitution of polysorbates by plant-based emulsifiers: impact on vitamin D bioavailability and gut health in mice
    Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Mouse inflamation Panel Detection Antibodies from the kit LEGENDplex Mouse Inflammation Panel (13-plex) with V-botton Plate, biolegend, (Catalog number 740446) Anti-mouse IgG Horseradish Peroxidase linked (CliniScience, 01017-05) 3 nature portfolio | reporting sum m ary April 2023 Goat anti-mouse IgG Fab-HRP (Southern Biotech, Cliniscience 1015-05) anti-MUC2, Santa Cruz Biotechnology, Cat#sc-15334; AB_2146667 anti-VVA, Vector Labs, B-1235-2 Mouse antibodies from the kit Mouse Lipocalin-2/NGAL DuoSet ELISA, Bio-Techne SAS, Catalog #: DY1857 Validation Mouse inflamation Panel Detection Antibodies :Validated by the manufacturer Anti-mouse IgG Horseradish Peroxidase linked : Validated by manufacturer Goat anti-mouse IgG Fab-HRP : Validated by manufacturer anti-MUC2 and anti-VVA validated by manufacturer and in the lab for specific conditions Mouse antibodies from the kit Mouse Lipocalin-2/NGAL DuoSet ELISA, validated by manufacturer Eukaryotic cell lines Policy information about cell lines and Sex and Gender in Research Cell line source(s) Caco2-TC7, HEK-TLR4 and HEK-TLR5 Authentication For Caco2-TC7 Morphology check by microscope, growth curve analysis, control number of passages For HEK-TLR4 and HEK-TLR5 Indirect authentication through the use of selection agents and the creation of a standard range to validate TLR4 or TLR5 expression. ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Substitution of polysorbates by plant-based emulsifiers: impact on vitamin D bioavailability and gut health in mice
    Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Mouse inflamation Panel Detection Antibodies from the kit LEGENDplex Mouse Inflammation Panel (13-plex) with V-botton Plate, biolegend, (Catalog number 740446) Anti-mouse IgG Horseradish Peroxidase linked (CliniScience, 01017-05) 3 nature portfolio | reporting sum m ary April 2023 Goat anti-mouse IgG Fab-HRP (Southern Biotech, Cliniscience 1015-05) anti-MUC2, Santa Cruz Biotechnology, Cat#sc-15334; AB_2146667 anti-VVA, Vector Labs, B-1235-2 Mouse antibodies from the kit Mouse Lipocalin-2/NGAL DuoSet ELISA, Bio-Techne SAS, Catalog #: DY1857 Validation Mouse inflamation Panel Detection Antibodies :Validated by the manufacturer Anti-mouse IgG Horseradish Peroxidase linked : Validated by manufacturer Goat anti-mouse IgG Fab-HRP : Validated by manufacturer anti-MUC2 and anti-VVA validated by manufacturer and in the lab for specific conditions Mouse antibodies from the kit Mouse Lipocalin-2/NGAL DuoSet ELISA, validated by manufacturer Eukaryotic cell lines Policy information about cell lines and Sex and Gender in Research Cell line source(s) Caco2-TC7, HEK-TLR4 and HEK-TLR5 Authentication For Caco2-TC7 Morphology check by microscope, growth curve analysis, control number of passages For HEK-TLR4 and HEK-TLR5 Indirect authentication through the use of selection agents and the creation of a standard range to validate TLR4 or TLR5 expression. ..

    Biomarker Discovery:

    Article Title: Substitution of polysorbates by plant-based emulsifiers: impact on vitamin D bioavailability and gut health in mice
    Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Mouse inflamation Panel Detection Antibodies from the kit LEGENDplex Mouse Inflammation Panel (13-plex) with V-botton Plate, biolegend, (Catalog number 740446) Anti-mouse IgG Horseradish Peroxidase linked (CliniScience, 01017-05) 3 nature portfolio | reporting sum m ary April 2023 Goat anti-mouse IgG Fab-HRP (Southern Biotech, Cliniscience 1015-05) anti-MUC2, Santa Cruz Biotechnology, Cat#sc-15334; AB_2146667 anti-VVA, Vector Labs, B-1235-2 Mouse antibodies from the kit Mouse Lipocalin-2/NGAL DuoSet ELISA, Bio-Techne SAS, Catalog #: DY1857 Validation Mouse inflamation Panel Detection Antibodies :Validated by the manufacturer Anti-mouse IgG Horseradish Peroxidase linked : Validated by manufacturer Goat anti-mouse IgG Fab-HRP : Validated by manufacturer anti-MUC2 and anti-VVA validated by manufacturer and in the lab for specific conditions Mouse antibodies from the kit Mouse Lipocalin-2/NGAL DuoSet ELISA, validated by manufacturer Eukaryotic cell lines Policy information about cell lines and Sex and Gender in Research Cell line source(s) Caco2-TC7, HEK-TLR4 and HEK-TLR5 Authentication For Caco2-TC7 Morphology check by microscope, growth curve analysis, control number of passages For HEK-TLR4 and HEK-TLR5 Indirect authentication through the use of selection agents and the creation of a standard range to validate TLR4 or TLR5 expression. ..

    Microscopy:

    Article Title: Substitution of polysorbates by plant-based emulsifiers: impact on vitamin D bioavailability and gut health in mice
    Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Mouse inflamation Panel Detection Antibodies from the kit LEGENDplex Mouse Inflammation Panel (13-plex) with V-botton Plate, biolegend, (Catalog number 740446) Anti-mouse IgG Horseradish Peroxidase linked (CliniScience, 01017-05) 3 nature portfolio | reporting sum m ary April 2023 Goat anti-mouse IgG Fab-HRP (Southern Biotech, Cliniscience 1015-05) anti-MUC2, Santa Cruz Biotechnology, Cat#sc-15334; AB_2146667 anti-VVA, Vector Labs, B-1235-2 Mouse antibodies from the kit Mouse Lipocalin-2/NGAL DuoSet ELISA, Bio-Techne SAS, Catalog #: DY1857 Validation Mouse inflamation Panel Detection Antibodies :Validated by the manufacturer Anti-mouse IgG Horseradish Peroxidase linked : Validated by manufacturer Goat anti-mouse IgG Fab-HRP : Validated by manufacturer anti-MUC2 and anti-VVA validated by manufacturer and in the lab for specific conditions Mouse antibodies from the kit Mouse Lipocalin-2/NGAL DuoSet ELISA, validated by manufacturer Eukaryotic cell lines Policy information about cell lines and Sex and Gender in Research Cell line source(s) Caco2-TC7, HEK-TLR4 and HEK-TLR5 Authentication For Caco2-TC7 Morphology check by microscope, growth curve analysis, control number of passages For HEK-TLR4 and HEK-TLR5 Indirect authentication through the use of selection agents and the creation of a standard range to validate TLR4 or TLR5 expression. ..

    Control:

    Article Title: Substitution of polysorbates by plant-based emulsifiers: impact on vitamin D bioavailability and gut health in mice
    Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Mouse inflamation Panel Detection Antibodies from the kit LEGENDplex Mouse Inflammation Panel (13-plex) with V-botton Plate, biolegend, (Catalog number 740446) Anti-mouse IgG Horseradish Peroxidase linked (CliniScience, 01017-05) 3 nature portfolio | reporting sum m ary April 2023 Goat anti-mouse IgG Fab-HRP (Southern Biotech, Cliniscience 1015-05) anti-MUC2, Santa Cruz Biotechnology, Cat#sc-15334; AB_2146667 anti-VVA, Vector Labs, B-1235-2 Mouse antibodies from the kit Mouse Lipocalin-2/NGAL DuoSet ELISA, Bio-Techne SAS, Catalog #: DY1857 Validation Mouse inflamation Panel Detection Antibodies :Validated by the manufacturer Anti-mouse IgG Horseradish Peroxidase linked : Validated by manufacturer Goat anti-mouse IgG Fab-HRP : Validated by manufacturer anti-MUC2 and anti-VVA validated by manufacturer and in the lab for specific conditions Mouse antibodies from the kit Mouse Lipocalin-2/NGAL DuoSet ELISA, validated by manufacturer Eukaryotic cell lines Policy information about cell lines and Sex and Gender in Research Cell line source(s) Caco2-TC7, HEK-TLR4 and HEK-TLR5 Authentication For Caco2-TC7 Morphology check by microscope, growth curve analysis, control number of passages For HEK-TLR4 and HEK-TLR5 Indirect authentication through the use of selection agents and the creation of a standard range to validate TLR4 or TLR5 expression. ..

    Selection:

    Article Title: Substitution of polysorbates by plant-based emulsifiers: impact on vitamin D bioavailability and gut health in mice
    Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Mouse inflamation Panel Detection Antibodies from the kit LEGENDplex Mouse Inflammation Panel (13-plex) with V-botton Plate, biolegend, (Catalog number 740446) Anti-mouse IgG Horseradish Peroxidase linked (CliniScience, 01017-05) 3 nature portfolio | reporting sum m ary April 2023 Goat anti-mouse IgG Fab-HRP (Southern Biotech, Cliniscience 1015-05) anti-MUC2, Santa Cruz Biotechnology, Cat#sc-15334; AB_2146667 anti-VVA, Vector Labs, B-1235-2 Mouse antibodies from the kit Mouse Lipocalin-2/NGAL DuoSet ELISA, Bio-Techne SAS, Catalog #: DY1857 Validation Mouse inflamation Panel Detection Antibodies :Validated by the manufacturer Anti-mouse IgG Horseradish Peroxidase linked : Validated by manufacturer Goat anti-mouse IgG Fab-HRP : Validated by manufacturer anti-MUC2 and anti-VVA validated by manufacturer and in the lab for specific conditions Mouse antibodies from the kit Mouse Lipocalin-2/NGAL DuoSet ELISA, validated by manufacturer Eukaryotic cell lines Policy information about cell lines and Sex and Gender in Research Cell line source(s) Caco2-TC7, HEK-TLR4 and HEK-TLR5 Authentication For Caco2-TC7 Morphology check by microscope, growth curve analysis, control number of passages For HEK-TLR4 and HEK-TLR5 Indirect authentication through the use of selection agents and the creation of a standard range to validate TLR4 or TLR5 expression. ..

    Expressing:

    Article Title: Substitution of polysorbates by plant-based emulsifiers: impact on vitamin D bioavailability and gut health in mice
    Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Mouse inflamation Panel Detection Antibodies from the kit LEGENDplex Mouse Inflammation Panel (13-plex) with V-botton Plate, biolegend, (Catalog number 740446) Anti-mouse IgG Horseradish Peroxidase linked (CliniScience, 01017-05) 3 nature portfolio | reporting sum m ary April 2023 Goat anti-mouse IgG Fab-HRP (Southern Biotech, Cliniscience 1015-05) anti-MUC2, Santa Cruz Biotechnology, Cat#sc-15334; AB_2146667 anti-VVA, Vector Labs, B-1235-2 Mouse antibodies from the kit Mouse Lipocalin-2/NGAL DuoSet ELISA, Bio-Techne SAS, Catalog #: DY1857 Validation Mouse inflamation Panel Detection Antibodies :Validated by the manufacturer Anti-mouse IgG Horseradish Peroxidase linked : Validated by manufacturer Goat anti-mouse IgG Fab-HRP : Validated by manufacturer anti-MUC2 and anti-VVA validated by manufacturer and in the lab for specific conditions Mouse antibodies from the kit Mouse Lipocalin-2/NGAL DuoSet ELISA, validated by manufacturer Eukaryotic cell lines Policy information about cell lines and Sex and Gender in Research Cell line source(s) Caco2-TC7, HEK-TLR4 and HEK-TLR5 Authentication For Caco2-TC7 Morphology check by microscope, growth curve analysis, control number of passages For HEK-TLR4 and HEK-TLR5 Indirect authentication through the use of selection agents and the creation of a standard range to validate TLR4 or TLR5 expression. ..



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